HBsAg ELISA (Blood Banking)

HBsAg ELISA (Blood Banking)

Accurate Detection of Hepatitis B Surface Antigen (HBsAg)

The HBsAg ELISA kit from Vanguard Diagnostics offers a fast, accurate, and reliable method for detecting Hepatitis B surface antigen in human serum or plasma. This enzyme immunoassay is designed for the qualitative detection of HBsAg, aiding in the diagnosis of both acute and chronic Hepatitis B Virus (HBV) infections.

Pack Size: 96 Tests

Shelf Life: 18 months

Storage Conditions: 2° to 8° C

Key Features

• High Sensitivity & Specificity: Utilises monoclonal anti-HBs antibodies to ensure accurate detection of HBsAg, minimising false positives and negatives.

• Rapid & Reliable Results: Designed for shorter reaction times compared to conventional assays, facilitating efficient testing workflows.

• User-Friendly Design: Compatible with standard microwell ELISA readers, making it suitable for various laboratory settings.

• Comprehensive Kit Components: Includes all necessary reagents and materials for conducting the assay, ensuring convenience and consistency.

Intended Use

The HBsAg ELISA kit is intended for the qualitative detection of Hepatitis B surface antigen in human serum or plasma. It serves as a crucial tool in screening blood donations and diagnosing HBV infections, thereby preventing the transmission of Hepatitis B.

For detailed test procedures, precautions, and result interpretation, please refer to the Instructions for Use (IFU).

Assay Principle

The HBsAg ELISA test employs a sandwich enzyme-linked immunosorbent assay methodology:

1. Sample Addition: Serum or plasma samples are added to microwells pre-coated with anti-HBs antibodies, allowing any present HBsAg to bind.

2. Detection Antibody Addition: An enzyme-conjugated detection antibody is introduced, binding to the captured HBsAg.

3. Substrate Reaction: A substrate solution is added, leading to a colorimetric change proportional to the amount of HBsAg in the sample.

4. Result Interpretation: The intensity of the color change is measured using an ELISA plate reader, indicating the presence or absence of HBsAg.

Kit Components

Each HBsAg ELISA kit includes:

• 96-Well Microtiter Plate: Pre-coated with monoclonal anti-HBs antibodies.

• Enzyme-Conjugated Detection Antibody: Facilitates the detection of bound HBsAg.

• Positive & Negative Controls: Ensure assay validity and reliability.

• Wash Buffer & Diluent: For sample and reagent preparation.

• Substrate & Stop Solutions: Used in the colorimetric detection process.

• Comprehensive Instruction Manual: Provides detailed guidelines for assay execution and result interpretation.

Storage & Stability

• Storage Temperature: Maintain at 2°C to 8°C. Do not freeze.

• Shelf Life: 18 months from the date of manufacture.

• Handling Precautions: Keep reagents tightly sealed and store away from direct light to maintain assay integrity.

Performance Characteristics

• High Sensitivity & Specificity: Capable of detecting low levels of HBsAg in serum or plasma, ensuring accurate results.

• Reduced Cross-Reactivity: Minimises false positives by reducing interference from other substances.

• Efficient Testing Process: Shorter incubation times enhance laboratory efficiency without compromising result quality.

Frequently Asked Questions (FAQs)

What is the purpose of the HBsAg ELISA kit?

The kit is used for the qualitative detection of Hepatitis B surface antigen in human serum or plasma, aiding in the diagnosis of HBV infections and ensuring the safety of blood donations.

How does the HBsAg ELISA test work?

It utilises a sandwich ELISA method where HBsAg, if present in the sample, is captured by immobilised antibodies and then detected using an enzyme-conjugated secondary antibody, leading to a measurable colorimetric change.

What sample type is required for this test?

The assay requires human serum or plasma samples for accurate detection of HBsAg.

What is the shelf life of the HBsAg ELISA kit?

The kit has a shelf life of 18 months when stored appropriately at 2°C to 8°C.

How accurate is the HBsAg ELISA test?

The test demonstrates high sensitivity and specificity, utilising monoclonal antibodies to ensure precise detection and minimise false results.

What is the HBsAg ELISA test price?

Pricing varies based on order quantity:

• Up to 5 kits: Rs. 1,700 per kit + 12% GST

• 6 to 10 kits: Rs. 1,650 per kit + 12% GST

• More than 10 kits: Rs. 1,600 per kit + 12% GST

For bulk orders or institutional pricing, please contact us directly.

Why Choose Vanguard’s HBsAg ELISA Kit?

• Regulatory Compliance: Manufactured under stringent quality control standards, ensuring compliance with international diagnostic regulations.

• Optimised for Blood Banks: Specifically designed to meet the rigorous demands of blood screening facilities, ensuring the safety of blood products.

• Cost-Effective Solution: Offers a competitive HBsAg ELISA test price without compromising on quality or performance.

Ready to enhance your Hepatitis B screening capabilities?

Order Now to ensure reliable detection with Vanguard’s trusted ELISA technology.

For further inquiries or bulk orders, please contact us.