HCV ELISA Microwell Test – Vanguard Diagnostics

Accurate Microwell ELISA for Detection of HCV Antibodies

The HCV ELISA kit from Vanguard Diagnostics is a microwell enzyme-linked immunosorbent assay designed for the qualitative detection of antibodies to Hepatitis C Virus (anti-HCV) in human serum or plasma. This test is intended for in vitro diagnostic use and aids in screening individuals for HCV infection.

Developed for high sensitivity and specificity, the test uses recombinant HCV antigens (core, NS3, NS4, and NS5) to ensure broad epitope recognition, making it ideal for blood banks, hospitals, and clinical laboratories.

Intended Use

The HCV ELISA kit is used for the qualitative detection of HCV antibodies in human serum or plasma. It is an in vitro diagnostic test for screening individuals who may have been exposed to Hepatitis C Virus.

Principle of the Test

This is a third-generation indirect ELISA. Recombinant HCV antigens are coated on the wells of a microwell plate. If anti-HCV antibodies are present in the sample, they bind to these immobilised antigens. An enzyme-conjugated secondary antibody is added to form a sandwich complex. After substrate addition, a colour change indicates the presence of anti-HCV antibodies, measurable at 450 nm.

Test Kit Contents

Key Features

• Third-generation ELISA using multiple HCV antigens
• High sensitivity and specificity
• Rapid and clear colourimetric results
• Suitable for automated and manual testing platforms
• Designed for routine HCV antibody screening

Performance Characteristics

• Sensitivity: >99% (based on evaluation panel data)
• Specificity: >99%
• Reading wavelength: 450 nm
• Sample type: Human serum or plasma (no haemolysis or lipaemia)

Storage and Stability

• Store at 2°C to 8°C
• Do not freeze reagents
• Stable until expiry date printed on the label
• Do not use components beyond their shelf life

Precautions

• For professional in vitro diagnostic use only
• Handle all specimens as potentially infectious
• Do not interchange reagents between lots
• Avoid microbial contamination of reagents
• Use PPE and dispose of waste as per biohazard protocols

Application

The HCV ELISA kit is intended for use in:

• Blood banks
• Hospital laboratories
• Clinical diagnostic centres
• Preventive screening programmes

Ordering Information

• Product: HCV ELISA Microwell Kit
• Format: 96 tests per kit
• Sample Type: Human serum/plasma
• Reading: 450 nm
• Shelf Life: As per label
• Storage: 2–8°C

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Frequently Asked Questions (FAQs)

What is the HCV ELISA test used for?

The HCV ELISA test is used to detect antibodies against Hepatitis C Virus (anti-HCV) in human serum or plasma, helping to identify HCV exposure.

Is this a third-generation HCV ELISA?

Yes, the test uses third-generation recombinant antigens (core, NS3, NS4, NS5) for improved sensitivity and early detection.

What is the sample requirement for the test?

The test requires human serum or plasma. Avoid haemolysed or contaminated samples.

What is the reading wavelength for this test?

The absorbance should be measured at 450 nm using a microplate reader.

How should the HCV ELISA kit be stored?

Store the kit between 2°C and 8°C, and do not freeze. All reagents must be used within their expiry dates.