LDL Cholesterol Clinical Chemistry

LDL Cholesterol Reagents – Direct Assay Kit for Clinical Chemistry

This liquid reagent kit enables direct quantitative measurement of LDL Cholesterol in human serum or plasma, based on an enzymatic end-point method with quinone pigment formation. The colour intensity correlates directly with LDL-C concentration and is read at 578 nm. Ideal for use in both semi-auto and fully automated analysers, this kit supports rapid and reliable lipid profiling in routine diagnostics.

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Key Features

• In-vitro direct assay for LDL Cholesterol in serum/plasma
• Enzymatic reaction method with endpoint reading at 578 nm
• Low sample volume (5 µl)
• Total reaction time: 8 minutes (5 + 3)
• Linear range: Up to 1000 mg/dl
• Compatible with semi-auto and autoanalysers
• Reagents and calibrator stable at 2–8°C

Principle of the Test

The reagent eliminates non-LDL cholesterol and isolates LDL-C for specific reaction. Cholesterol esters undergo enzymatic hydrolysis and oxidation using Cholesterol Esterase and Cholesterol Oxidase. In the presence of TOOS and 4-AA, the resulting hydrogen peroxide reacts with Catalase to form a quinone pigment measurable at 578 nm.

Intended Use

For the in-vitro determination of direct LDL Cholesterol concentration in human serum and plasma samples. Suitable for routine lipid panel analysis.

Kit Components

• R1 Reagent: 375 µl
• R2 Reagent: 125 µl
• Direct LDL Calibrator (reconstitute with 1 ml distilled water)
• Product Insert

Test Parameters

Specimen Requirements

Use fresh, non-haemolysed serum. Bring all reagents to room temperature before testing. Avoid contamination or repeated freezing.

FAQs – LDL Cholesterol Reagents

What are LDL cholesterol reagents used for?

They are used to quantitatively estimate low-density lipoprotein cholesterol (LDL-C) in human serum or plasma.

What is the method used for measuring LDL Cholesterol?

The kit uses an endpoint colorimetric method with enzymatic reactions and final absorbance measured at 578 nm.

Is this kit suitable for automated analysers?

Yes, the kit is designed for compatibility with both semi-auto and auto analysers.

What is the sample volume required per test?

Only 5 µl of serum or plasma is needed.

How should the calibrator be used?

Reconstitute the calibrator with 1 ml distilled water and store at 2–8°C. Use within 7 days.

What is the linear range of the assay?

The test is linear up to 1000 mg/dl. For higher concentrations, dilute and re-test.

How many tests are possible with this kit?

Each pack contains 2 × 25 mL reagents, suitable for approximately 50 tests depending on usage volume.