Typhoid RT PCR Kit

Accurate Detection of Salmonella typhi and paratyphi Using Real-Time PCR

The Vanguard VANSCAN Typhoid RT PCR Kit is a highly sensitive and specific solution for the qualitative detection of Salmonella typhi and paratyphi in human whole blood. Designed for use in molecular diagnostic laboratories, this real-time PCR test delivers rapid and reliable results using DNA amplification targeting the omp gene.

With minimal sample preparation and optimised thermal cycling, this typhoid RT PCR kit is ideal for early detection in clinical settings where traditional blood culture methods fall short in speed and sensitivity.

Key Features

• Target Pathogen: Salmonella typhi and paratyphi
• Target Gene: omp gene
• Detection Method: Real-time PCR (RT-PCR)
• Fluorophores Used: FAM (target DNA), HEX (internal control)
  
  
• Result Interpretation:
• Positive: Ct ≤ 38 in both FAM and HEX channels
• Negative: Ct ≤ 38 in HEX, no FAM signal or Ct > 38
• Invalid: Ct ≥ 38 or absent in HEX

• Sample Type: Whole blood (EDTA), 2–3 mL
  
  
• Storage:
• Sample: 2–8 °C for ≤48 hours or –80 °C for long-term storage
• Reagents: As per kit label; avoid >4 freeze–thaw cycles
  
  
• Pack Size: 24 tests per kit
• Reaction Volume: 20 μL

Kit Components

• RT-PCR Master Mix
• Primer–Probe Mix
• Internal Control
• Positive Control
• Nuclease-Free Water
• Enrichment Buffer (optional for low-load samples)
• PCR Tubes
• IFU (Instructions for Use)

Advantages Over Conventional Typhoid Testing

• High Sensitivity: Detects as low as ~1–10 CFU/mL
• Faster Turnaround: Results available within a few hours
• Validated Accuracy: 100% sensitivity and 97% specificity (multi-centre validation)
• Low Interference: Accurate results even in complex clinical samples

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Frequently Asked Questions (FAQs)

What is the principle of the Typhoid RT PCR Kit?

The test amplifies the omp gene of Salmonella typhi/paratyphi using RT-PCR and detects it via fluorescent probes (FAM for target, HEX for internal control).

How is the result interpreted?

A positive result is indicated by Ct ≤ 38 in both FAM and HEX channels. A negative result shows Ct ≤ 38 in HEX and no signal in FAM. An invalid result requires repeat testing.

Can I enrich the bacterial sample before testing?

Yes, for low-burden samples, an enrichment buffer step is available. Blood is incubated with buffer, spun down, and the pellet is used for DNA extraction.

What sample type is required?

2–3 mL of EDTA-treated whole blood is recommended. Store samples at 4 °C for up to 48 hours or freeze at –80 °C for longer periods.

Is this kit compatible with standard PCR machines?

Yes, it is compatible with most real-time PCR instruments using FAM and HEX channels and requires no passive reference dye.