VANSCAN Typhoid RT PCR

VANSCAN Typhoid RT PCR Kit

Real-Time PCR Detection of Salmonella Typhi and Paratyphi

The VANSCAN Typhoid RT PCR Kit is a qualitative, in vitro diagnostic test for the detection of Salmonella typhi and Salmonella paratyphi DNA in human whole blood. Designed for use in diagnostic laboratories, the kit employs real-time PCR amplification with fluorescent probe detection, enabling early, specific identification of typhoid pathogens directly from clinical samples.

This kit is part of our molecular diagnostics product line, supporting rapid, evidence-based diagnostics in clinical settings.

Key Features

• Detects both S. typhi and S. paratyphi with high specificity
• Real-time PCR with fluorescence-based probe detection (FAM and HEX/VIC channels)
• Detection limit: ≤10 pg of bacterial DNA, equivalent to approximately 1–10 CFU/mL
• Optimised primer-probe mixes for improved accuracy and reduced cross-reactivity
• Validated on commonly used RT PCR platforms
• Rapid protocol and minimal handling
• For laboratory use only

Kit Components

• Primer-probe mix (1.2 mL)
• Enzyme mix (0.6 mL)
• Positive control (50 µL)
• Nuclease-free water
• PCR-grade reaction tubes
• Instructions for Use (Download here)

Sample Requirements and Storage

• Sample Type: Human whole blood
• Storage: All reagents must be stored at –20°C
• Stability: Valid for 24 months from manufacturing date
• Handling: Reagents should be thawed on ice and protected from light. Avoid more than 4 freeze–thaw cycles.

Clinical Use and Applications

The VANSCAN Typhoid RT PCR Kit is intended for:

• Early molecular detection of typhoid and paratyphoid fevers
• Confirmatory testing in cases where serology (e.g., Widal test) or culture results are inconclusive
• Reducing diagnostic delays during outbreak situations
• Supporting targeted antibiotic stewardship by enabling evidence-based treatment decisions

To explore other molecular testing solutions, view our complete molecular diagnostics segment.

Frequently Asked Questions (FAQs)

What does the VANSCAN Typhoid RT PCR Kit detect?

The kit detects DNA from Salmonella typhi and Salmonella paratyphi using a real-time PCR assay with fluorescent probes.

What is the detection limit of this typhoid PCR test?

It can detect ≤10 picograms of bacterial DNA, which corresponds to 1–10 CFU/mL of whole blood.

What type of sample is required?

The test is validated for human whole blood samples.

Which PCR platforms are compatible with this kit?

Compatible instruments must support FAM and HEX/VIC dye channels, including common real-time PCR machines.

How should the kit be stored and handled?

Store at –20°C. Once thawed, reagents should be used promptly and should not undergo more than 4 freeze–thaw cycles.

Is this kit suitable for routine diagnostic use?

Yes, the VANSCAN Typhoid RT PCR Kit is intended for clinical laboratory use and supports routine detection of typhoid pathogens.