Bilirubin Total & Direct Reagent Kit

Product Overview

Bilirubin Total & Direct Reagent Kit is designed for the quantitative estimation of total and direct bilirubin in human serum and plasma using the modified Jendrassik & Grof method.

The assay is suitable for semi-automated and fully automated chemistry analysers and is used in clinical chemistry workflows involving bilirubin measurement and liver function testing. For general information on bilirubin testing, see MedlinePlus: Bilirubin Blood Test.

For related clinical chemistry testing, explore SGOT (AST) Reagents, SGPT (ALT) Reagents and Total Protein Reagent.

Key Features:

• Tests per pack: Available in packs suitable for 500 tests.
• Methodology: Modified Jendrassik and Grof.
• Detection: Measures both total and direct bilirubin with high sensitivity. 
• Sample type: Human serum and plasma
• Reagent stability: Stable up to 12 months at 2–8°C; working reagent stable for 7 days.
• Instrument compatibility: Compatible with a wide range of semi-auto and fully automated chemistry analysers.
• Linearity:
•              Total bilirubin: Up to 20 mg/dL
•               Direct bilirubin: Up to 10 mg/dL
• Intended use: Ideal for liver function tests, jaundice screening, and hepatic disorder monitoring.

How It Works:

The assay relies on the diazotisation of bilirubin in the presence of diazotised sulphanilic acid. Caffeine acts as an accelerator in total bilirubin estimation, while direct bilirubin reacts directly. The intensity of the pink-coloured complex formed is proportional to the bilirubin concentration and is measured photometrically.

Pack Contents:

• Sulphanilic Acid Reagent
• Sodium Nitrite Reagent
• Caffeine Reagent
• Tartrate Reagent
• Detailed IFU (Instruction for Use)

Related Products:

For comprehensive liver and metabolic health screening, explore:

• SGOT/AST Reagent Kit – for monitoring liver enzyme levels

• SGPT/ALT Reagent Kit – used alongside bilirubin testing

• Total Protein Reagent Kit – assess nutritional and hepatic health

Frequently Asked Questions

What is the purpose of bilirubin reagent testing?

This test helps detect and monitor liver function by measuring bilirubin levels. High levels may indicate liver disease, bile duct obstruction, or haemolysis.

What’s the difference between total and direct bilirubin?

Total bilirubin includes both unconjugated (indirect) and conjugated (direct) bilirubin. The direct test measures only the water-soluble form, which the liver has processed.

How many tests does the bilirubin reagent kit support?

This pack supports up to 500 tests, depending on analyser configuration and sample throughput.

Can I use this with any analyser?

Yes, the kit is compatible with most automated chemistry analysers and semi-auto biochemistry platforms.