CRP Immunoturbidity method

CRP Immunoturbidity Kit – Accurate Quantification of C-Reactive Protein

The CRP Immunoturbidity Kit is a turbidimetric assay designed for the quantitative determination of C-Reactive Protein (CRP) in human serum or plasma. CRP is a non-specific acute-phase protein that increases in concentration during inflammatory processes, making this kit essential for diagnostic laboratories and healthcare providers monitoring inflammation and associated conditions.

Key Features

• High Sensitivity: Detects CRP levels as low as 2 mg/L, enabling early identification of inflammatory responses.

• Wide Measuring Range: Accurate quantification of CRP concentrations up to 150 mg/L.

• Rapid Results: Provides timely information to assist in clinical decision-making.

Intended Use

This kit is intended for the quantitative measurement of CRP in human serum or plasma, aiding in the evaluation of tissue injury, inflammatory disorders, and related diseases.

Assay Principle

The assay utilizes a turbidimetric method where latex particles coated with anti-CRP antibodies aggregate in the presence of CRP, causing an increase in turbidity. This change is measured photometrically, and the increase in absorbance is proportional to the CRP concentration in the sample.

Kit Components

• Reagent 1 (CRP Buffer): 40 mL

• Reagent 2 (CRP Latex): 10 mL

• Reagent 3 (CRP Calibrator): 1 mL

• Product Insert: Detailed instructions for use

Preparation of Working Reagent

Mix 4 parts of Reagent 1 (CRP Buffer) with 1 part of Reagent 2 (CRP Latex) gently, avoiding foaming. The working reagent is stable for 3 days when stored at 2-8°C.

Storage and Stability

• Unopened Kit: Stable up to the expiration date when stored at 2-8°C.

• Working Reagent: Stable for 3 days at 2-8°C.

• Onboard Stability: Reagents are stable for 10 days on the analyzer at 2-10°C.

Specimen Collection and Storage

Collect fresh serum samples and store at 2-8°C for up to 48 hours. For longer storage, freeze the samples. Avoid using contaminated or hemolyzed samples.

Performance Characteristics

• Sensitivity: Detects CRP concentrations as low as 2 mg/L.

• Linearity: Accurate measurements up to 150 mg/L. Samples with higher concentrations should be diluted with saline (1:4), and the final result multiplied by 5.

Quality Control

Each assay run should include normal and abnormal control samples to ensure accuracy and reliability of results.

Ordering Information

• Pack Size: 50 mL

• Shelf Life: 18 months

• Storage: 2° - 8°C

• Price: ₹1,600.00 per kit (up to 5 kits per order; discounts available for larger quantities)

For more details or to place an order, please visit our product page.

Frequently Asked Questions (FAQs)

1. What is the CRP Immunoturbidity Kit used for?

The kit is used for the quantitative measurement of C-Reactive Protein (CRP) in human serum or plasma, aiding in the assessment of inflammatory conditions and tissue injury.

2. How does the CRP Immunoturbidity assay work?

The assay measures the turbidity increase caused by the aggregation of latex particles coated with anti-CRP antibodies in the presence of CRP, with the change in absorbance being proportional to the CRP concentration.

3. What is the sensitivity of this kit?

The kit can detect CRP concentrations as low as 2 mg/L, allowing for early detection of inflammation.

4. How should samples be stored before testing?

Serum samples can be stored at 2-8°C for up to 48 hours. For longer storage periods, samples should be frozen to maintain integrity.

5. What is the shelf life of the CRP Immunoturbidity Kit?

The kit has a shelf life of 18 months when stored at 2-8°C, as indicated on the product label.