Total Protein Reagents

Accurate, Efficient, and Reliable Protein Estimation

Vanguard Diagnostics’ Total Protein Reagents offer a robust solution for the quantitative determination of total protein in human serum and plasma, using the Biuret method. This ready-to-use kit ensures dependable performance for clinical laboratories and diagnostic centres seeking consistent, high-quality results.

Key Features:

• Biuret Method: Gold standard for protein quantification, with excellent reproducibility.
• Working Reagent Format: Ready-to-use liquid reagent—no preparation required.
• Wide Measuring Range: 1–12 g/dL, suitable for various clinical contexts.
• Dual Wavelength Compatibility: 540 nm or 578 nm, adaptable to standard photometers and autoanalysers.
• Excellent Linearity & Stability: Offers stable readings for up to 30 days at 2–8°C once opened.

This kit is designed for both manual and automated analysers, supporting labs looking to scale testing volumes without compromising accuracy. It is CE-certified and validated for in-vitro diagnostic use only.

For laboratories using automated systems like the 3-part hematology analyser, the ready-to-use format reduces error and saves time.

Kit Components:

• Biuret Reagent (1 × 100 mL or 5 × 100 mL)
• Standard Solution (6 g/dL)

Refer to the IFU for specific volumes per configuration.

Intended Use

This kit is intended for the quantitative estimation of total protein in human serum and plasma. It is designed for use in clinical laboratories by trained professionals and should not be used for self-diagnosis or home testing.

Storage & Stability

• Store at 2–8°C
• Do not freeze
• Once opened, stable for 30 days if stored properly and handled aseptically

Clinical Relevance

Total protein testing is essential for evaluating liver function, kidney disorders, nutritional status, and chronic diseases. Abnormal protein levels may indicate liver disease, kidney dysfunction, or infections.

Pair with cholesterol reagents or uric acid reagents for comprehensive metabolic panels.

Frequently Asked Questions

What is the Biuret method used in this kit?

The Biuret method detects peptide bonds in proteins, producing a purple-coloured complex measurable via spectrophotometry. It is one of the most widely used methods for total protein estimation due to its simplicity and accuracy.

How should the Total Protein Reagents be stored?

Store the reagents between 2–8°C. Do not freeze. Ensure reagents are sealed after use to maintain stability.

Is the kit suitable for automated analysers?

Yes, the Total Protein Reagents are compatible with both manual and fully automated systems, including photometers and semi-auto/fully auto chemistry analysers.

Can I use this kit alongside other biochemical tests?

Yes, it is commonly used as part of a routine biochemical panel, especially when paired with SGPT, SGOT, and bilirubin reagents.

Where can I get more information?

Visit our Contact Us page to reach our technical support team.