Uric Acid Reagents – For Accurate Quantitative Estimation in Serum or Plasma

Vanguard Diagnostics’ Uric Acid Reagents are designed for the quantitative determination of uric acid in human serum or plasma, using a precise enzymatic colorimetric method. This diagnostic-grade formulation enables reliable detection for routine clinical biochemistry, supported by robust reagent stability, minimal interference, and clear procedural steps aligned with international standards.

Why Choose Vanguard’s Uric Acid Reagents?

• Based on Uricase-POD Methodology: A proven enzymatic reaction where uricase converts uric acid to allantoin and hydrogen peroxide, followed by a Trinder reaction to produce a measurable red chromogen.
• High Accuracy and Linearity: Linear up to 20 mg/dL, ideal for most clinical ranges. Results remain reliable even at borderline pathophysiological concentrations.
• Convenient Two-Reagent System: Easy-to-use reagents include phosphate buffer with enzymes, 4-aminoantipyrine, TOOS, peroxidase, and stabilisers.
• Optimised for Manual and Automated Analysers: Protocols are compatible with semi-auto and fully automated systems used in clinical labs.

Test Parameters

Intended Use

This uric acid test reagent kit is intended for in vitro diagnostic use by qualified personnel in medical laboratories. It supports the monitoring and diagnosis of conditions such as gout, kidney dysfunction, metabolic syndrome, and chemotherapy-related uric acid imbalances.

Reagent Composition

• Reagent 1 (R1): Phosphate buffer, uricase, TOOS, 4-AAP, sodium azide (preservative)
• Reagent 2 (R2): Peroxidase, phosphate buffer, stabilisers

Both reagents are ready-to-use and do not require further preparation.

Procedure Overview

1. Mixing: Add R1 and R2 to sample/test tubes as per volume instructions.
2. Incubation: Maintain at 37°C for 5 minutes (or room temperature for 10 minutes).
3. Measurement: Read absorbance at 520 nm against blank within 30 minutes.

Full procedural details are available in the Uric Acid IFU PDF.

Other Related Reagents You Might Find Useful

• Creatinine Reagents – For assessing kidney function alongside uric acid levels
• Bilirubin Reagents – Total and direct bilirubin estimation in serum
• Amylase Reagents – Enzymatic method for pancreatic and salivary amylase

These links help complete the test panel for renal and metabolic evaluations using clinical biochemistry reagents.

FAQs

1. What are uric acid reagents used for?

Uric acid reagents are used to measure uric acid levels in blood samples. This helps in diagnosing and monitoring gout, renal function disorders, and conditions related to purine metabolism.

2. How should the reagents be stored?

Store at 2–8°C, away from light. Do not freeze the reagents.

3. What type of analyser can I use for this test?

The kit is compatible with both manual and automated analysers that can measure absorbance at 520 nm.

4. Can I use plasma samples?

Yes, both serum and plasma samples can be used, provided they are free from haemolysis.

5. What is the shelf life of the uric acid reagent kit?

Reagents are stable until the expiration date printed on the label, provided storage conditions are followed.