HIV ELISA (Blood Banking)

HIV ELISA 4th Generation Kit 

Early Detection of HIV-1 p24 Antigen & HIV-1/2 Antibodies

The Vanguard Diagnostics HIV ELISA 4th Generation Kit is a highly sensitive and specific in vitro diagnostic solution for the qualitative detection of HIV-1 p24 antigen and antibodies to HIV-1 (including group O) and HIV-2 in human serum or plasma.

By detecting both antigen and antibodies, this HIV ELISA kit significantly shortens the diagnostic window period, making it ideal for blood bank screening, diagnostic labs, and early detection strategies.

Pack Size: 96 Tests

Storage: 2–8°C

Shelf Life: 18 months

📄 Download the IFU

Key Features

• 4th Generation Detection: Antigen + antibody capture for early-stage diagnosis

• High Sensitivity & Specificity: Validated to meet international benchmarks

• Optimised for Manual & Automated ELISA Systems

• Internal Controls Included for accurate results

• Colourimetric Detection at 450 nm

Kit Contents

• Microwell plate pre-coated with HIV antigens and antibodies

• HIV-1 Ag Positive Control

• HIV-1 and HIV-2 Positive Controls

• Negative Control

• Biotin-Conjugate Reagent

• HRP-Conjugate Reagent

• Wash Buffer (20x)

• Chromogen A & B

• Stop Solution

• Sealing film, instruction manual

Assay Overview (Simplified Protocol)

1. Add 100 µL of sample + 20 µL Biotin-Conjugate

2. Incubate 60 mins at 37°C

3. Wash

4. Add HRP-Conjugate (100 µL), incubate 30 mins

5. Wash

6. Add substrate (Chromogen A+B), incubate 15 mins

7. Add Stop Solution

8. Read absorbance at 450 nm

Refer to the full IFU for complete step-by-step instructions.

Performance Characteristics

• Sensitivity: 100%

• Specificity: 99.76%

• No cross-reactivity with specimens positive for HAV, HBV, HCV, HTLV, CMV, TP

• Validated using WHO International Reference Panels

Ideal Use Cases

• Blood Banks
• Hospitals
• Government Screening Programs
• Clinical & Diagnostic Labs
•  NGOs running HIV awareness and screening campaigns

Frequently Asked Questions (FAQs)

What is a 4th generation HIV ELISA test?

It’s an advanced HIV ELISA test that detects both HIV-1 p24 antigen and antibodies to HIV-1 and HIV-2. This enables detection as early as 2–3 weeks post-infection, reducing the window period significantly.

What is included in the HIV ELISA kit?

The kit includes pre-coated wells, positive/negative controls, conjugates, substrates, wash buffer, and stop solution — everything needed to run 96 tests.

What is the HIV test kit price?

Pricing varies based on quantity and region. To get accurate institutional or bulk pricing for the HIV test kit, please contact us here.

Is this kit approved for diagnostic use in India?

Yes. The HIV ELISA kit complies with Indian diagnostic regulations and is manufactured under ISO-certified quality systems.

Can the kit be used with an automated ELISA system?

Yes, the reagents are compatible with most open ELISA processors.

Where can I find more technical details?

All test principles, sample preparation, and calculation methods are detailed in the official Instructions for Use (IFU).

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