Triglycerides Reagents – Accurate, Liquid Stable Testing for Lipid Profiles

Vanguard Diagnostics’ triglycerides reagents are designed for the quantitative determination of triglycerides in human serum or plasma. Using an enzymatic, colourimetric method, this liquid stable kit delivers precise and reliable lipid profiling — essential for identifying hyperlipidaemia, cardiovascular risk, and metabolic disorders in clinical settings.

Suitable for both automated and semi-automated analysers, the reagents are supplied ready to use, ensuring seamless lab integration with minimal preparation time.

Key Features

• Method: Enzymatic colourimetric (LPL, GK, GPO, POD)
• Application: Quantitative determination of triglycerides in serum/plasma
• Linearity: Up to 885 mg/dL (10 mmol/L)
• Read Mode: End-point, absorbance at 500 nm
• Stability: 18 months at 2–8°C unopened; 21 days after reconstitution
• Interference: No significant effect from haemoglobin (≤500 mg/dL), bilirubin (≤20 mg/dL), or lipemic samples (intralipid ≤1000 mg/dL)
• Pack Size: 2 × 50 mL

Clinical Utility

Triglycerides measurement is a core part of routine lipid profiling. Elevated triglyceride levels are associated with increased risk of cardiovascular events, pancreatitis, and metabolic syndrome. These triglycerides reagents offer high specificity and sensitivity, ensuring diagnostic accuracy even in high-throughput environments.

Kit Components

• Reagent 1 (Buffer): 2 × 50 mL
• Reagent 2 (Enzyme): 1 × 12 mL
• Calibrator
• Product insert with IFU

Storage & Stability

• Unopened Kit: Stable for 18 months at 2–8°C
• Working Reagent: Stable for 21 days at 2–8°C or 3 days at 15–25°C
• Onboard Stability: Up to 10 days on the analyser at 2–10°C
• Sample Storage: Serum/plasma stable for 7 days refrigerated or 3 months frozen at –20°C

Performance Characteristics

• Linearity: Up to 885 mg/dL
• Precision:

• Intra-assay CV < 2%

• Inter-assay CV < 3%

• No significant interference observed from haemoglobin, bilirubin, or lipemia

Frequently Asked Questions

What is the intended use of the Triglycerides Reagents?

These reagents are used for in-vitro quantitative measurement of triglycerides in human serum or plasma.

What’s the assay method used?

The kit uses an enzymatic colourimetric method involving lipoprotein lipase, glycerol kinase, glycerol-3-phosphate oxidase, and peroxidase to generate a measurable colour change proportional to triglyceride concentration.

Can the kit be used with automated analysers?

Yes, it is compatible with most automated and semi-automated clinical chemistry analysers.

How long are the reagents stable?

The unopened kit is stable for 18 months at 2–8°C. Working reagent remains stable for 21 days when refrigerated.

What types of samples can be tested?

The assay is intended for use with human serum or plasma samples.

Explore Related Biochemistry Reagent Kits

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• Cholesterol Reagents – For total cholesterol measurement using the CHOD-PAP method
• HDL Cholesterol Reagents – For high-density lipoprotein (HDL) cholesterol estimation
• LDL Cholesterol Reagents – For low-density lipoprotein (LDL) cholesterol testing
• Uric Acid Reagents – Supports metabolic and renal function diagnostics
• SGPT Reagents – ALT liver enzyme quantification