Triglycerides Reagents – Accurate, Liquid Stable Testing for Lipid Profiles

Product Overview

Triglycerides Reagents are intended for the in-vitro quantitative determination of triglycerides in human serum and plasma. The kit uses a GPO-POD enzymatic method, involving lipase, glycerol kinase (GK), glycerol-3-phosphate oxidase (GPO) and peroxidase (POD).

The reagents are ready to use and suitable for a range of automated and semi-automated clinical chemistry analysers. The assay uses an end-point reaction, with automated applications read at 546 nm and a stated linearity of 800 mg/dL.

For general information on triglyceride testing, see MedlinePlus: Triglycerides Test.

For related lipid testing products, explore Cholesterol Reagents, HDL Cholesterol Reagents and LDL Cholesterol Reagents.

Key Features

• Method: Enzymatic colourimetric (LPL, GK, GPO, POD)
• Application: Quantitative determination of triglycerides in serum/plasma
• Linearity: 800 mg/dL (10 mmol/L)
• Read Mode: End-point, absorbance at 546 nm
• Stability: 18 months at 2–8°C unopened; 21 days after reconstitution
• Interference: No significant effect from haemoglobin (≤500 mg/dL), bilirubin (≤20 mg/dL), or lipemic samples (intralipid ≤1000 mg/dL)
• Pack Size: 2 × 50 mL

Clinical Utility

Triglycerides measurement is a core part of routine lipid profiling. Elevated triglyceride levels are associated with increased risk of cardiovascular events, pancreatitis, and metabolic syndrome. These triglycerides reagents offer high specificity and sensitivity, ensuring diagnostic accuracy even in high-throughput environments.

Kit Components

• Reagent 1 (Buffer): 2 × 50 mL
• Reagent 2 (Enzyme): 1 × 12 mL
• Calibrator
• Product insert with IFU

Storage & Stability

• Unopened Kit: Stable for 18 months at 2–8°C
• Working Reagent: Stable for 21 days at 2–8°C or 3 days at 15–25°C
• Onboard Stability: Up to 10 days on the analyser at 2–10°C
• Sample Storage: Serum/plasma stable for 7 days refrigerated or 3 months frozen at –20°C

Performance Characteristics

• Linearity: 800 mg/dL
• Precision:
• Intra-assay CV < 2%
• Inter-assay CV < 3%
• No significant interference observed from haemoglobin, bilirubin, or lipemia

Frequently Asked Questions

What is the intended use of the Triglycerides Reagents?

These reagents are used for in-vitro quantitative measurement of triglycerides in human serum or plasma.

What’s the assay method used?

The kit uses an enzymatic colourimetric method involving lipoprotein lipase, glycerol kinase, glycerol-3-phosphate oxidase, and peroxidase to generate a measurable colour change proportional to triglyceride concentration.

Can the kit be used with automated analysers?

Yes, it is compatible with most automated and semi-automated clinical chemistry analysers.

How long are the reagents stable?

The unopened kit is stable for 18 months at 2–8°C. Working reagent remains stable for 21 days when refrigerated.

What types of samples can be tested?

The assay is intended for use with human serum or plasma samples.

Explore Related Biochemistry Reagent Kits

Build a comprehensive lipid or metabolic panel with other compatible reagents from Vanguard:

• Cholesterol Reagents – For total cholesterol measurement using the CHOD-PAP method
• HDL Cholesterol Reagents – For high-density lipoprotein (HDL) cholesterol estimation
• LDL Cholesterol Reagents – For low-density lipoprotein (LDL) cholesterol testing
• Uric Acid Reagents – Supports metabolic and renal function diagnostics
• SGPT Reagents – ALT liver enzyme quantification